Gambaran Pemeriksaan IgG dan IgM Demam Berdarah Dengue pada Pasien di Rumah Sakit Umum Pancaran Kasih Kota Manado

Authors

  • Novatania Makagansa Universitas Sam Ratulangi
  • Wulan P J Kaunang Universitas Sam Ratulangi
  • Grace D Kandou Universitas Sam Ratulangi

DOI:

https://doi.org/10.36312/ej.v6i3.3187

Keywords:

Gambaran Pemeriksaan IgG, IgM, Demam Berdarah Dengue

Abstract

Demam berdarah dengue merupakan infeksi virus dengue yang di tularkan ke manusia melalui gigitan nyamuk aedes aegypti dan aedes albopictus yang di temukan di daerah beriklim tropis dan subtropik. Menurut Badan Pusat Statistik Sulawesi Utara tahun 2024, Kota Manado menduduki urutan ke empat dalam jumlah kasus Demam berdarah dengue terbanyak. Penyakit ini dapat di tegakkan berdasarkan gejala klinis dan pemeriksaan laboratorium. Salah satu pemeriksaan laboratorium yakni Pemeriksaan Serologis Immunoglobin G (IgG) dan Immunoglobulin M (IgM) anti dengue. Penelitian bertujuan untuk menggambarkan pemeriksaan IgG dan IgM pada pasien demam berdarah dengue di Rumah Sakit Umum Pancaran Kasih Kota Manado. Jenis penelitian adalah penelitian kuantitatif deskriptif dengan desain penelitian cross sectional-study. Sampel dalam penelitian ini adalah seluruh pasien yang dilakukan pemeriksaan IgG dan IgM anti dengue dalam periode januari – desember 2024 yang berjumlah 151 pasien. Hasil penelitian menunjukkan bahwa perempuan lebih banyak dilakukan pemeriksaan IgG dan IgM anti dengue berjumlah 80 orang (53,0%) dengan mayoritas hasil pemeriksaan IgG + IgM – ( 33 orang) mengindikasikan infeksi sekunder, sedangkan laki laki 71 orang (47,0%) dengan hasil pemeriksaan yang paling dominan adalah hasil IgG – IgM – (38 orang) mengindikasikan infeksi awal. Kelompok usia 6-9 tahun memiliki jumlah yang paling dominan, berjumlah 44 orang (29,1% ) dan kelompok usia 19-59 berjumlah 43 orang (28,5%). Mayoritas pasien pada kedua kelompok usia ini memiliki hasil IgG – IgM –. Kelompok Usia 6-9 tahun (19 orang) dan kelompok usia 19-59 (25 orang).

Description of IgG And IgM Examination for Dengue Hemorrhagic Fever in Patients at Pancaran Kasih General Hospital, Manado City

Abstract

Dengue fever is a dengue virus infection transmitted to humans through the bites of Aedes aegypti and Aedes albopictus mosquitoes, found in tropical and subtropical climates. According to the North Sulawesi Central Statistics Agency (BPS) in 2024, Manado City ranked fourth in terms of the number of dengue fever cases. This disease can be diagnosed based on clinical symptoms and laboratory tests. One of the laboratory tests is the serological examination of anti-dengue immunoglobulin G (IgG) and anti-dengue immunoglobulin M (IgM). This study aimed to describe IgG and IgM testing in dengue fever patients at Pancaran Kasih General Hospital, Manado City. This study was a descriptive quantitative study with a cross-sectional design. The sample consisted of all 151 patients who underwent anti-dengue IgG and IgM testing between January and December 2024. The results showed that females (80) underwent anti-dengue IgG and IgM testing more frequently, with the majority of IgG + IgM – results (33) indicating secondary infection. Males (71) were male, with the most dominant IgG – IgM – results (38) indicating primary infection. The 6-9 age group had the highest number of patients, with 44 (29.1%), and the 19-59 age group had 43 (28.5%). The majority of patients in both age groups had IgG – IgM – results. Age group 6-9 years (19 people) and age group 19-59 (25 people).

Downloads

Download data is not yet available.

References

Badan Pusat Statistik. (2024). Kasus penyakit menurut kabupaten/kota dan jenis penyakit di Provinsi Sulawesi Utara, 2023. https://sulut.bps.go.id/id/statistics-table/3/YTA1Q1ptRmhUMEpXWTBsQmQyZzBjVzgwUzB4aVp6MDkjMw==

Bellinda Putri Kolondam, Jeini Ester Nelwan, G. D. K. (2020). Gambaran perilaku masyarakat tentang upaya pencegahan penyakit demam berdarah dengue. Journal of Public Health and Community Medicine, 1.

Bone, T., Kaunang, W. P. J., & Langi, F. (2021). Hubungan antara curah hujan, suhu udara dan kelembaban dengan kejadian demam berdarah dengue di kota Manado tahun 2015–2020. Kesmas, 10(5), 36–45.

Bone, T., Kaunang, W.P.J. and Langi, F. (2021) „Hubungan antara curah hujan, suhu udara dan kelembaban dengan kejadian demam berdarah dengue di kota manado tahun 2015-2020?, Kesmas, 10(5), pp. 36–45.

Changal, K., Raina, A., Raina, A., Raina, M., Bashir, R., Latief, M., … & Changal, Q. (2016). Differentiating secondary from primary dengue using igg to igm ratio in early dengue: an observational hospital based clinico-serological study from north india. BMC Infectious Diseases, 16(1). https://doi.org/10.1186/s12879-016-2053-6

Charisma, A. M., Farida, E. A., & Anwari, F. (2020). Diagnosis dengue melalui deteksi antibodi immunoglobulin G spesifik dalam sampel urine dengan teknik ELISA. ASPIRATOR - Journal of Vector-borne Disease Studies, 12(1), 11–18. https://doi.org/10.22435/asp.v12i1.2366 .

Conde, J., Silva, E., Barbosa, A., & Mohana?Borges, R. (2017). The complement system in flavivirus infections. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.00213

Goethals, O., Kaptein, S., Kesteleyn, B., Bonfanti, J., Wesenbeeck, L., Bardiot, D., … & Loock, M. (2023). Blocking ns3–ns4b interaction inhibits dengue virus in non-human primates. Nature, 615(7953), 678-686. https://doi.org/10.1038/s41586-023-05790-6

Halstead, S. and Wilder?Smith, A. (2019). Severe dengue in travellers: pathogenesis, risk and clinical management. Journal of Travel Medicine, 26(7). https://doi.org/10.1093/jtm/taz062

Hayes, C., Dauner, A., Valks, A., Forshey, B., Long, K., Thaisomboonsuk, B., … & Wu, S. (2015). Multicountry prospective clinical evaluation of two enzyme-linked immunosorbent assays and two rapid diagnostic tests for diagnosing dengue fever. Journal of Clinical Microbiology, 53(4), 1092-1102. https://doi.org/10.1128/jcm.03042-14

John, A. and Rathore, A. (2019). Adaptive immune responses to primary and secondary dengue virus infections. Nature Reviews Immunology, 19(4), 218-230. https://doi.org/10.1038/s41577-019-0123-x

Katzelnick, L., Montoya, M., Gresh, L., Balmaseda, Á., & Harris, E. (2016). Neutralizing antibody titers against dengue virus correlate with protection from symptomatic infection in a longitudinal cohort. Proceedings of the National Academy of Sciences, 113(3), 728-733. https://doi.org/10.1073/pnas.1522136113

Kaunang, W. P. J., et al. (2025). Public health surveillance: A foundation for disease control and health promotion. https://www.researchgate.net/publication/393157164

Kaunang, W.P., Tulung, M., & Salaki, C. (2014). Phenotypical morphometry variation of Aedes aegypti in Manado. Scientific Research Journal (SCIRJ), II(XII), 15–22. https://www.scirj.org/papers-1214/scirj-P1214217.pdf

Kaunang, W.P.. (2022a) Surveilans Kesehatan Masyarakat. Edited by T.P. Media.Bandung: CV. Patra Media Grafindo Bandung.

Kaunang, W.P.. . and Kabak, V. (2024) Demam Berdarah Dengue (DBD). Available at: https://www.researchgate.net/profile/Wulan-Kaunang-2/2.

Kaunang, W.P.. et al. (2025) „Public Health Surveillance : A Foundation for Disease Control and Health Public Health Surveillance : A Foundation for Disease Control and Health Promotion?, (June), pp. 0–8. Available at: https://www.researchgate.net/publication/393157164_Public_Health_Surveillance_A_Foundation_for_Disease_Control_and_Health_Promotion/link/68623965e4632b045dc88755/download?_tp=eyJjb250ZXh0Ijp7ImZpcn N0UGFnZSI6InByb2ZpbGUiLCJwYWdlIjoicHVibGljYXRpb24iLCJwc mV2aW91c1BhZ2UiOiJwcm9maWxlIn19.

Kaunang, W.P.J. (2024) Surveilans Kesehatan Masyarakat: Strategi Pengendalian Demam Berdarah Dengue. Edited by B. Murti. Yogyakarta: Deepublish.

Kaunang, W.P.J., Tulung, M. and Salaki, C. (2014) „Phenotypical Morphometry Variation of Aedes Aegyti in Manado?, Scientific Research Journal (SCIRJ, II(Xii), pp. 15–22. Available at: https://www.scirj.org/papers- 1214/scirj-P1214217.pdf.

Kaunang, W.P.. (2022b) Vector borne disease (dbd). Available at: https://www.researchgate.net/publication/366544533_MINI_SKRIPSI_VECTOR_BORNE_DISEASE_DBD.

Kembuan, G. (2020). Dengue serology in indonesian covid-19 patients: coinfection or serological overlap?. Idcases, 22, e00927. https://doi.org/10.1016/j.idcr.2020.e00927

Kemenkes. (2024). Waspadae, kenaikan kasus DBD belum mencapai puncak. Biro Komunikasi dan Pelayanan Publik, Kementerian Kesehatan RI. https://kemkes.go.id/id/waspada-kenaikan-kasus-dbd-belum-mencapai-puncak

Kementerian Kesehatan. (2024). Waspadae, kenaikan kasus DBD belum mencapai puncak. https://kemkes.go.id/id/waspada-kenaikan-kasus-dbd-belum-mencapai-puncak .

Khairinisa, G. (2025). Upaya pencegahan dan pengendalian vektor demam berdarah melalui edukasi dan deteksi serta pengukuran indeks ovitrap nyamuk Aedes aegypti, 1(1), 1–11.

Kharisma, S.B. (2024). Gambaran hasil pemeriksaan IgG dan IgM pada pasien demam berdarah dengue di Rumah Sakit Islam Jombang. Jurnal Kesehatan Islam Indonesia, 12(1), 43–49.

Kurnia, N. (2022). Pemeriksaan serologis IgG-IgM pada pasien demam berdarah dengue di RSUD Dr. Chasan Basoeri Ternate periode Oktober–Desember 2021. JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan), 7(2), 117. https://doi.org/10.30829/jumantik.v7i2.11294

Liu, L., Chen, C., Tsai, C., Lin, P., Hsu, M., Huang, B., … & Tsai, J. (2020). Evaluation of rapid diagnostic tests to detect dengue virus infections in taiwan. Plos One, 15(9), e0239710. https://doi.org/10.1371/journal.pone.0239710

Luo, S., Cui, W., Li, C., Ling, F., Fu, T., Liu, Q., … & Sun, J. (2018). Seroprevalence of dengue igg antibodies in symptomatic and asymptomatic individuals three years after an outbreak in zhejiang province, china. BMC Infectious Diseases, 18(1). https://doi.org/10.1186/s12879-018-3000-5

Lyski, Z. and Messer, W. (2019). Approaches to interrogating the human memory b-cell and memory-derived antibody repertoire following dengue virus infection. Frontiers in Immunology, 10. https://doi.org/10.3389/fimmu.2019.01276

Mahasurya, I. G. A. D., Lestari, A. A. W., & Yasa, I. W. P. S. (2017). Gambaran pemeriksaan serologi IgM-IgG anti-dengue pasien terinfeksi virus dengue di Rumah Sakit Surya Husada Denpasar Bali pada periode Desember 2013 sampai Mei 2014. E-Jurnal Medika, 6(1), 1–6.

Manthalkar, P. and Peerapur, B. (2015). Sero diagnosis of dengue virus infection using elisa in patients with suspected dengue infection. Journal of Evolution of Medical and Dental Sciences, 4(62), 10824-10828. https://doi.org/10.14260/jemds/2015/1562

Marvianto, D., Yuliyanti, N., Murdani, A., & Febrian, R. (2023). Infeksi dengue sekunder: Patofisiologi, diagnosis, dan implikasi klinis. Majalah Kedokteran Bandung, 50(2), 70–74.

Nabeshima, T., Tun, M., Th?y, N., H?ng, N., Quynh, L., Hasebe, F., … & Takamatsu, Y. (2023). An outbreak of a novel lineage of dengue virus 2 in vietnam in 2022. Journal of Medical Virology, 95(11). https://doi.org/10.1002/jmv.29255

Olajiga, O.M., Taku, N.A., & Simons, R. (2022). Association of dengue infection with anti-alpha-gal antibodies. Frontiers in Immunology, 13, 1021016. https://doi.org/10.3389/fimmu.2022.1021016

Paomey, V. C., Nelwan, J. E., & Kaunang, W. P. J. (2019). Sebaran penyakit demam berdarah dengue berdasarkan ketinggian dan kepadatan penduduk di Kecamatan Malalayang Kota Manado tahun 2019. Jurnal KESMAS, 8(6), 521–527.

Paomey, V.C., Nelwan, J.E. and Kaunang, W.P.J. (2019) „Sebaran Penyakit Demam Berdarah Dengue Berdasarkan Ketinggian Dan Kepadatan Penduduk Di Kecamatan Malalayang Kota Manado Tahun 2019?, Jurnal KESMAS, 8(6), pp. 521–527.

Paputungan, M.H. and Kaunang, W.P.J. (2017) „Faktor Risiko Yang Berhubungan Dengan Kejadian Demam Berdarah Dengue Di Wilayah Kerja Puskesmas Gogagoman Kecamatan Kotamobagu Barat Kota Kotamobagu?, E- Journal, pp. 37–42. Available at: https://ejournal.unsrat.ac.id/v3/index.php/jikmu/article/view/9408 .

R.S, R., Ronald Ottay, I. and Kaunang, W.P.J. (2015) „Pemetaan Penyebaran Penyakit Demam Berdarah Dengue Dengan Geographic Information System (Gis) Di Kota Kotamobagu?, Jurnal Kedokteran Komunitas dan Tropik, III(2), pp. 48–56. Available at: https://www.academia.edu/download/79100590/7335.pdf .

Ranawaka, R., Jayamanne, C., Dayasiri, K., Samaranayake, D., Sandakelum, U., Hathagoda, W., … & Wickramasinghe, P. (2021). Effect of prior symptomatic dengue infection on dengue haemorrhagic fever (dhf) in children. Journal of Tropical Medicine, 2021, 1-5. https://doi.org/10.1155/2021/8842799

RI, K. K. (2017). Pedoman pencegahan dan pengendalian demam berdarah dengue di Indonesia. Jakarta.

Rini, D. and Aryati, A. (2018). Sd dengue duo® (ns1, igg, igm) rapid test dalam menunjang diagnosis infeksi virus dengue. Indonesian Journal of Clinical Pathology and Medical Laboratory, 16(2), 97-101. https://doi.org/10.24293/ijcpml.v16i2.980

Roy, S. and Bhattacharjee, S. (2021). Dengue virus: epidemiology, biology, and disease aetiology. Canadian Journal of Microbiology, 67(10), 687-702. https://doi.org/10.1139/cjm-2020-0572

Santi, N., Anwar, C., & Sunarsih, E. (2023). Epidemiologi, biologi, patogenesis, manifestasi klinis, dan diagnosis infeksi virus dengue di indonesia: kajian literatur komprehensif. Jurnal Ilmiah Permas Jurnal Ilmiah Stikes Kendal, 13(4), 1179-1188. https://doi.org/10.32583/pskm.v13i4.1235

Sasmono, R., Kalalo, L., Trismiasih, S., Denis, D., Yohan, B., Hayati, R., … & Haryanto, S. (2019). Multiple introductions of dengue virus strains contribute to dengue outbreaks in east kalimantan, indonesia, in 2015–2016. Virology Journal, 16(1). https://doi.org/10.1186/s12985-019-1202-0

Setya, G., & Putri, A. (2023). Hubungan hasil pemeriksaan IgM dan IgG anti-dengue di RSUD Dr. Gondo Suwarno Semarang. Borneo Journal of Medical Laboratory Technology, 6, 531–536. https://journal.umpr.ac.id/index.php/bjmlt/article/download/7384/4706/31425

Syed, F., Arif, M., Mansoor, V., Usman, M., & Arif, S. (2024). Evolving spectrum of dengue: a two-year experience from a tertiary care hospital in pakistan. Cureus. https://doi.org/10.7759/cureus.53817

Tatura, S.N.N., Oroh, M.C.E., Lintong, L.A., et al. (2021). Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia. International Journal of Infectious Diseases, 106, 185–196. https://doi.org/10.1016/j.ijid.2021.03.065

Torres-Flores, J., Reyes?Sandoval, A., & Salazar, M. (2022). Dengue vaccines: an update. Biodrugs, 36(3), 325-336. https://doi.org/10.1007/s40259-022-00531-z

Trisnadewi, N.N.L., & Wande, I.N. (2016). Pola serologi IgM dan IgG pada infeksi demam berdarah dengue (DBD) di Rumah Sakit Umum Pusat Sanglah, Denpasar, Bali bulan Agustus sampai September 2014. E-Jurnal Medika, 5(8), 1–5.

Tsai, H., Tsai, Y., Lin, I., Kuo, P., Chang, K., Chen, J., … & Wang, J. (2016). Validation and application of a commercial quantitative real-time reverse transcriptase-pcr assay in investigation of a large dengue virus outbreak in southern taiwan. Plos Neglected Tropical Diseases, 10(10), e0005036. https://doi.org/10.1371/journal.pntd.0005036

Tumey, A., Kaunang, W.P.J. and Asrifuddin, A. (2020) „hubungan variabilitas iklim dengan kejadian demam berdarah dengue (dbd) di kabupaten kepulauan talaud tahun 2018 - juni 2020?, Kesmas, 9(7), pp. 16–27. Available at: https://ejournal.unsrat.ac.id/index.php/kesmas/article/view/31607.

Vickers, I., Harvey, K., Brown, M., Nelson, K., Ducasse, M., & Lindo, J. (2015). The performance of the sd bioline dengue duo® rapid immunochromatographic test kit for the detection of ns1 antigen, igm and igg antibodies during a dengue type 1 epidemic in jamaica. Journal of Biomedical Science, 22(1). https://doi.org/10.1186/s12929-015-0164-9

WHO. (2024a). Dengue and severe dengue. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue

WHO. (2024b). WHO launches global strategic plan to fight rising dengue and other aedes borne arboviral diseases. https://www.who.int/news/item/03-10-2024-who-launches-global-strategic-plan-to-fight-rising-dengue-and-other-aedes-borne-arboviral-diseases

Downloads

Published

2025-09-30

Issue

Section

Articles

How to Cite

Makagansa, N., Kaunang, W. P. . J., & Kandou, G. D. (2025). Gambaran Pemeriksaan IgG dan IgM Demam Berdarah Dengue pada Pasien di Rumah Sakit Umum Pancaran Kasih Kota Manado. Empiricism Journal, 6(3), 1559-1568. https://doi.org/10.36312/ej.v6i3.3187