Explorasi Potensi Mikrobium Bahan Pangan sebagai Biodetergent
DOI:
https://doi.org/10.36312/jar.v4i1.3064Keywords:
Mikrobium, Bahan Pangan, Biodetergent.Abstract
Penelitian ini bertujuan untuk mengevaluasi daya cuci mikrobium dari bahan pangan lokal, menilai potensinya dalam mengurangi penggunaan detergen kimia, serta mengidentifikasi kemungkinan keberadaan enzim-enzim penting yang berperan dalam proses pembersihan. Mikrobium dari berbagai bahan pangan dikultur, dan supernatan ekstrak kasar (mengandung enzim) digunakan dalam uji pencucian dengan bahan pengotor berupa minyak zaitun. Pengukuran daya cuci didasarkan pada pengurangan residu minyak zaitun setelah proses pencucian. Efektivitas ekstrak kasar diuji pada tiga tingkat konsentrasi detergen: 0%, 0.5%, dan 1.0%, untuk mengevaluasi apakah mikrobium dapat mengurangi kebutuhan detergen kimiawi. Hasil menunjukkan bahwa mikrobium bahan pangan memiliki daya cuci signifikan, yang berbeda tergantung jenis bahan dan konsentrasi. Terdapat interaksi signifikan antara jenis bahan dan konsentrasi, yang menunjukkan bahwa efektivitas daya cuci tergantung pada kombinasi keduanya. Meskipun belum sepenuhnya menggantikan peran detergen, mikrobium bahan pangan menunjukkan potensi untuk meningkatkan efektivitas pencucian, terutama pada konsentrasi detergen rendah (0.5%). Uji aktivitas enzim menunjukkan bahwa mikrobium dari air kelapa memiliki aktivitas selulase, protease, dan amilase, sementara aktivitas lipase belum terdeteksi dalam uji ini.
References
Aehle, W. (2004). Enzymes in Industry: Production and Applications. Wiley-VCH.
Anandan, S., et al. (2007). Application of microbial enzymes in detergents. In Industrial Enzymes (pp. 373–392). Springer
Arechabala, B., Coiffard, L. J. M., Rivalland, P., & Coiffard, C. (1999).
Comparison of cytotoxicity of various surfactants tested on normal human fibroblasts cultured in vitro.
Cell Biology and Toxicology, 15(3), 143–151. https://doi.org/10.1023/A:1007065824564
Badmus SO, Amusa HK, Oyehan TA, Saleh TA. Environmental risks and toxicity of surfactants: overview of analysis, assessment, and remediation techniques. Environ Sci Pollut Res Int. 2021 Nov;28(44):62085-62104. doi: 10.1007/s11356-021-16483-w. Epub 2021 Sep 29. PMID: 34590224; PMCID: PMC8480275.
Banat, I. M., et al. (2010). Microbial biosurfactants production, applications and future potential. Applied Microbiology and Biotechnology, 87(2), 427–444.
Giagnorio, Mattia, Amelio Antonio, Grüttner H, Tiraferri A (2017). Environmental impacts of detergents and benefits of their recovery in the laundering industry. Journal of Cleaner Production. 54(15) 2017: 593-601
Kümmerer, K. (2001). Detergents and the environment. The Lancet, 358(9288), 344–345.
https://doi.org/10.1016/S0140-6736(01)05408-1
La Maestra S, D'Agostini F, Geretto M, Micale RT. Microbial-based cleaning products as a potential risk to human health: A review. Toxicol Lett. 2021 Dec 15;353:60-70. doi: 10.1016/j.toxlet.2021.09.013. Epub 2021 Oct 6.
Mulligan, C. N. (2005). Environmental applications for biosurfactants. Environmental Pollution, 133(2), 183–198. PMID: 34626814
Ron, E. Z., & Rosenberg, E. (2001). Natural roles of biosurfactants. Environmental Microbiology, 3(4), 229–236. https://doi.org/10.1046/j.1462-2920.2001.00190.xSedijani, P., Rasmi, D.A.C., Kusmiyati, K., & Anggriani, R.A. (2021). Powerful Lipolytic Activity of Fungi Isolated from Coconut and Avocado Flesh on Different pH and Temperature. Jurnal Penelitian Pendidikan IPA, 7(SpecialIssue), 365–369. https://doi.org/10.29303/jppipa.v7iSpecialIssue.1261
Subasinghe, R.M., A.D., Samarajeewa, M., Meier, G., Coleman, H., Clouthier, J., Crosthwait, A.F., Tayabali, R., Scroggins, P.S., Shwed, L.A and Beaud, E. Bacterial and fungal composition profiling of microbial based cleaning products. Food and Chemical Toxicology. Volume 116, Part A, June 2018, Pages 25-31
T. Arthur et al. (2012). Effects-driven chemical design: the acute toxicity of CO 2- triggered switchable surfactants to rainbow trout can be predicted from octanol- water partition coefficients, Green Chem.
Vandini A, Temmerman R, Frabetti A, Caselli E, Antonioli P, Balboni PG, Platano D., Branchini A, Mazzacane S (2014) Hard Surface Biocontrol in Hospitals Using Microbial-Based Cleaning Products. PLOS One 9: 1–13. [PMC free article] [PubMed] [Google Scholar]
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