Quantification of Phenolic Content in Bridelia Micrantha Bark Extract Using Folin-Ciocalteu Method
DOI:
https://doi.org/10.36312/jar.v4i1.3167Keywords:
Bridelia micrantha, phenolic content, Folin–Ciocalteu, antioxidant activity, medicinal plant, gallic acid equivalent, phytochemical screeningAbstract
The scientific validation of medicinal plants is essential to ensure the safety, efficacy, and reproducibility of traditional remedies in modern healthcare. Bridelia micrantha, although widely used in Southeast Asian and African ethnomedicine, has not been fully standardized despite claims of antioxidant and anti-inflammatory properties. This study determined the total phenolic content (TPC) of B. micrantha stem bark extract to support its pharmacological relevance. An experimental design was implemented using 80% methanol maceration for extraction, qualitative screening with FeCl?, and quantitative analysis via the Folin-Ciocalteu method. Absorbance was measured at 760 nm using UV-Vis spectrophotometry, with gallic acid as a calibration standard (r² = 0.9978). The extract showed a phenolic content of 22.14% gallic acid equivalent (GAE), indicating a substantial presence of antioxidant-related compounds. These findings confirm the traditional use of B. micrantha and highlight its potential for standardized herbal formulations. The study also bridges a gap between ethnobotanical knowledge and phytochemical evidence, contributing to the integration of traditional plant-based remedies into evidence-based healthcare systems.
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