Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities.

Authors

  • Dian Puspita Anggraini Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province, 66137
  • Devita Sulistiana Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province, 66137
  • Dwi Kameluh Agustina Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province 66137
  • Almira Ulimaz Agro-Industry Study Program, Department of Agricultural Industrial Technology, Politeknik Negeri Tanah Laut. Jl. A. Yani Km. 06, Panggung Village, Pelaihari District, Tanah Laut Regency, South Kalimantan Province, Indonesia, Postal code: 70815 http://orcid.org/0000-0003-1910-1752

DOI:

https://doi.org/10.36312/e-saintika.v4i2.170

Keywords:

Bacillus firmus, Mg2 , pectinase, purification.

Abstract

The purpose of study was to determine kinetic parameters and inhibitory effect of Mg2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of KM with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of Vm from both are different, that is 80,645 μg.ml-1.minute-1 and 62,112 μg.ml-1.minute-1. The conclusion that type of inhibitory pectinase with Mg2+ is a non-competitive inhibition. The inhibition constant value (Ki) is 26,84.

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References

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Published

2020-06-27

Issue

Vol. 4 No. 2 (2020): July

Section

Research Papers

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How to Cite

Anggraini, D. P., Sulistiana, D., Agustina, D. K., & Ulimaz, A. (2020). Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities. Jurnal Penelitian Dan Pengkajian Ilmu Pendidikan: E-Saintika, 4(2), 112-118. https://doi.org/10.36312/e-saintika.v4i2.170