Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities.

Authors

  • Dian Puspita Anggraini Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province, 66137, Indonesia
  • Devita Sulistiana Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province, 66137, Indonesia
  • Dwi Kameluh Agustina Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province 66137, Indonesia
  • Almira Ulimaz Agro-Industry Study Program, Department of Agricultural Industrial Technology, Politeknik Negeri Tanah Laut. Jl. A. Yani Km. 06, Panggung Village, Pelaihari District, Tanah Laut Regency, South Kalimantan Province, Indonesia, Postal code: 70815, Indonesia [email protected] ORCID: 0000-0003-1910-1752
Published
2020-06-27
Issue
Vol. 4 No. 2 (2020): July
Pages
112-118
Section
Research Papers

Abstract

The purpose of study was to determine kinetic parameters and inhibitory effect of Mg2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of KM with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of Vm from both are different, that is 80,645 μg.ml-1.minute-1 and 62,112 μg.ml-1.minute-1. The conclusion that type of inhibitory pectinase with Mg2+ is a non-competitive inhibition. The inhibition constant value (Ki) is 26,84.

Keywords

How to Cite

Dian Puspita Anggraini, Devita Sulistiana, Dwi Kameluh Agustina, Almira Ulimaz. (2020). Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities.. Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika, 4(2), 112-118. https://journal-center.litpam.com/index.php/e-Saintika/article/view/170

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How to Cite

Anggraini, D. P., Sulistiana, D., Agustina, D. K., & Ulimaz, A. (2020). Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities. Jurnal Penelitian Dan Pengkajian Ilmu Pendidikan: E-Saintika, 4(2), 112-118. https://doi.org/10.36312/e-saintika.v4i2.170