Development of rGO/Fe3O4 Composites as Glucose Biosensors

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Published
2021-07-30
Issue
Vol. 5 No. 2 (2021): July
Pages
168-174
Section
Natural and Applied Sciences

Abstract

The rGO/Fe3O4 composite is one type of composites that can be used as a biosensor material, especially glucose sensors. The main ingredients of the composite synthesis are graphite and iron sand. The synthesis process of Fe3O4 was done using the coprecipitation method, while the graphite oxidation process was accomplished using the modified Hummer's method. The composites were formed using the ex-situ wet mixing method. The formed iron sand and graphite were characterized using FTIR and XRD, and it was found that Fe3O4 was formed from the appearance of the Fe-O bond, the oxidation process of graphite was seen from the appearance of the C=O bond, and the detection of Fe peaks corresponded to the cubic crystal plane. Likewise, the composites formed were also characterized using FTIR and XRD for identification of the rGO/Fe3O4 composite formation. It was proven from the presence of Fe-O and C-O bonds and the appearance of an amorphous peak of rGO in the XRD results. The performance of the  rGO/Fe3O4 composites as the glucose biosensor was examined by varying the mass of Fe3O4 on the composite, using UV-Vis spectroscopy. The performance of the rGO/Fe3O4 composite biosensor in absorbing glucose reached optimum at a mass variation of 0.3 grams of Fe3O4, as demonstrated by by the lowest absorbance peak with an intensity of 0.0048 at a wavelength of 440 nm, corresponding to glucose entrappment of 7.1 mg/gram.

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How to Cite

Diah Hari Kusumawati, Rahayu Yudia Mufida. (2021). Development of rGO/Fe3O4 Composites as Glucose Biosensors. Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika, 5(2), 168-174. https://journal-center.litpam.com/index.php/e-Saintika/article/view/493

References

Chen, C.-Y., Yeh, K.-L., Aisyah, R., Lee, D.-J., & Chang, J.-S. (2011). Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review. Bioresource Technology, 102(1), 71–81. https://doi.org/10.1016/j.biortech.2010.06.159

He, C., Liu, J., Zhang, Q., & Wu, C. (2012). A novel stable amperometric glucose biosensor based on the adsorption of glucose oxidase on poly(methyl methacrylate)–bovine serum albumin core–shell nanoparticles. Sensors and Actuators B: Chemical, 166–167, 802–808. https://doi.org/10.1016/j.snb.2012.03.081

Hidayat, A., Setiadji, S., & Hadisantoso, E. P. (2019). Sintesis Oksida Grafena Tereduksi (rGO) dari Arang Tempurung Kelapa (Cocos nucifera). Al-Kimiya, 5(2), 68–73. https://doi.org/10.15575/ak.v5i2.3810

Karimi Pasandideh, E., Kakavandi, B., Nasseri, S., Mahvi, A. H., Nabizadeh, R., Esrafili, A., & Rezaei Kalantary, R. (2016). Silica-coated magnetite nanoparticles core-shell spheres (Fe3O4@SiO2) for natural organic matter removal. Journal of Environmental Health Science and Engineering, 14(1), 21. https://doi.org/10.1186/s40201-016-0262-y

Munasir, & Kusumawati, R. P. (2019). Synthesis and Characterization of Fe3O4@rGO Composite with Wet-Mixing (ex-situ) Process. Journal of Physics: Conference Series, 1171, 012048. https://doi.org/10.1088/1742-6596/1171/1/012048

Palanisamy, S., Karuppiah, C., & Chen, S.-M. (2014). Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode. Colloids and Surfaces B: Biointerfaces, 114, 164–169. https://doi.org/10.1016/j.colsurfb.2013.10.006

Qiu, C., Wang, X., Liu, X., Hou, S., & Ma, H. (2012). Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor. Electrochimica Acta, 67, 140–146. https://doi.org/10.1016/j.electacta.2012.02.011

Wang, B., Zhang, K., & Field, R. W. (2018). Optimization of aeration variables in a commercial large-scale flat-sheet MBR operated with slug bubbling. Journal of Membrane Science, 567, 181–190. https://doi.org/10.1016/j.memsci.2018.09.039

Wang, S., Tan, Y., Zhao, D., & Liu, G. (2008). Amperometric tyrosinase biosensor based on Fe3O4 nanoparticles–chitosan nanocomposite. Biosensors and Bioelectronics, 23(12), 1781–1787. https://doi.org/10.1016/j.bios.2008.02.014

Yu, L., Wu, H., Wu, B., Wang, Z., Cao, H., Fu, C., & Jia, N. (2014). Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing. Nano-Micro Letters, 6(3), 258–267. https://doi.org/10.1007/BF03353790

How to Cite

Kusumawati, D. H., & Mufida, R. Y. . (2021). Development of rGO/Fe3O4 Composites as Glucose Biosensors. Jurnal Penelitian Dan Pengkajian Ilmu Pendidikan: E-Saintika, 5(2), 168-174. https://doi.org/10.36312/esaintika.v5i2.493